Drug discovery is a multi-process that involves genomics, proteomics, and bioinformatics studies, which lead to the discovery of a new drug entity with a novel mechanism of action. A DNA Microarray is a test that allows for the comparison of thousands of genes at once. Clipping is a handy way to collect important slides you want to go back to later. Recent advances in proteomic technologies, including both mass spectrometry and protein microarrays, have enabled large-scale screening of proteins in tissues and serum from patients that are applicable for biomarker discovery. Drug metabolism in pharmaceutical research has traditionally focused on the well-defined aspects of absorption, distribution, metabolism and excretion, commonly-referred to ADME properties of a compound, particularly in the areas of metabolite identification, identification of drug metabolizing enzymes (DMEs) and associated metabolic pathways, and reaction mechanisms. Zhu Protein microarray (protein arrays or protein biochips) technology is a process of immobilizing numerous proteins on to a solid support and using miniaturized assays to describe protein interactions, that has been developed steadily in recent years. Important for toxicogenomic studies. Information from newer microarray technologies such as CGH, ChIP-on-chip, splice variants and microRNAs, combined with gene expression data, can be applied to the drug discovery process enabling many exciting applications. Microarray technology uses chips with attached DNA sequences as probes for gene expression. If you continue browsing the site, you agree to the use of cookies on this website. Protein Microarrays By Drug Discovery Trends Editor | November 12, 2007 SomaPlex slides from Protein Biotechnologies, for screening protein biomarkers in human tumors, are arrays prepared from a vast collection of total protein lysates derived from numerous human tissues representing a range of cancer subtypes. microarra y as a gene expression profiling tool microarra y as a comparativ e genomics tool disease diagnosis drug discovery toxicologic al research 22. Abstract: Protein microarray technology is one of the most powerful tools presently available for proteomic studies. Proteomic approach of drug discovery includes finding an unstable protein that is causing an undesirable We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. 3. Protein micro array 1. Earlier robots use solid pins, which can only print one feature before needing to collect more DNA from the well. The spots are printed on to the glass slide by a robot or are synthesised by the process of photolithography. If you continue browsing the site, you agree to the use of cookies on this website. DOI link for Functional Protein Microarrays in Drug Discovery. Now customize the name of a clipboard to store your clips. Each pin will spot a different grid on the array (Chapter 4). DNA Microarrays are one of the most effective invention ever developed. Principles of Clinical Pharmacology NIH, April 26, 2007 Role of FDA in Guiding Drug Development Carl Peck Center for Drug Development Science UCSF, UC-Washington Center – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 52a023-MjJmM role of nuclic acid microarray and protein microarray for drug discovery process 1.introduction about microarray technique and genomics 2.process of drug discovery 3.microarray techiques 4.microarray analysis in drug discovery 5.steps involved in the micro array analysis. Help to produce effective drugs 20. Chappell, and S.H. DNA microarrays are … They are essential for protein folding and stability and act as recognition elements in cell–cell and cell–matrix interactions. 1467-1476. The Critical Role of Surface Chemistry in Protein Microarrays . Protein microarrays allow for the simultaneous and rapid analysis of thousands of proteins in high-throughput (HT). Microarrays play an increasingly significant role in drug discovery. PROTEIN MICROARRAY K.KRUPA SAGAR M.Pharm 1st year 2. If you continue browsing the site, you agree to the use of cookies on this website. Microarray technology for genomic and proteomic research. Efficacy or potency, metabolic stability (half-life), and oral bioavailability are also improved in this step of the drug development process. Haplotype: a set of genetic determinants located on a single chromosome. drug targets, design more effective drugs, assessment of drug efficacy and patient response, i.e., to interfere with almost every steps in modern drug discovery process (Ahn et al., 2008). Glycans are key players in many biological processes. role of nuclic acid microarray and protein microarray for drug discovery process 1.introduction about microarray technique and genomics 2.process of drug disco… Looks like you’ve clipped this slide to already. See our Privacy Policy and User Agreement for details. Although nucleic acids may also be considered, their use as drug targets in drug discovery and structure based drug design has been limited due to various effects like toxicity, difficulty in achieving high specificity, etc. RNAprotect™ (Ambion) and RNAlater™ (Qiagen) are commercially available solutions that perfuse cells, and ‘freeze’ the cellular RNA profile. You can change your ad preferences anytime. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Microbial deterioration of textiles and paper, Customer Code: Creating a Company Customers Love, No public clipboards found for this slide. The technique has immense potential and promises to play a key role in furthering research in a number of fields, as discussed in this chapter. (c) The number of pins in the cassette can vary. (2007). The PMT amplifies the signal from each photon of light, which is converted into a digital value used to create the image representing signal intensity at each pixel position. View abstract . An update on using protein microarrays in drug discovery. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. This presentation will take you through the concept of microarray and its application in detail. Now customize the name of a clipboard to store your clips. They can be used for studying … •They allow simultaneous determination of a great variety of analytes from small amounts of samples within a single experiment. Other researchers use 5% phenol in ethanol which achieves the same result, prior to RNA extraction and purification. Looks like you’ve clipped this slide to already. (d) Most pins in modern use have a reservoir that holds sample and so can print multiple features – usually on different arrays – from a single visit to the well containing probe. See our Privacy Policy and User Agreement for details. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Protein microarrays are mainly applied to protein function studies, screening the production of antibodies and recombinant proteins , discovery of proteins implicated in disease or those that are potential drug targets, rapid detection or diagnosis of disease (18,19) screening for protein–protein, DNA–protein and enzyme–substrate interactions (20,21). Expert Opinion on Drug Discovery: Vol. Numerous types of protein microarrays have been widely and successfully applied for both basic biological studies and clinical researches, including those designed to characterize protein-protein, protein-nucleic acid, protein-drug/small molecule and antibody-antigen interactions. Role of Target Identification and Target Validation in Drug Discovery Process 1. A single-wavelength laser beam scanned back and forth across the array to excite an area representing a single pixel. If you continue browsing the site, you agree to the use of cookies on this website. Thus, being at the heart of medically relevant biological processes, glycans have come onto the scene and are considered hot sp Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. There are many different robots on the market; this is a Genetix spotting robot located at the Mouse Genetics Unit in Harwell, Oxfordshire. You can change your ad preferences anytime. PROTEIN MICROARRAYS Protein microarray is an emerging technology that provides a versatile platform for characterization of hundreds of thousands of proteins in a highly parallel and high-throughput way. The drug discovery process was beginning in 19 th century by John Langley in 1905 when he proposed the theory of respective substances. 11, pp. Taken together, these studies revealed that interesting proteins identified by proteomics in the intracellular pathways targeted by anticancer drugs can be classified into several categories, including proteins related to mitochondrial function, endoplasmic reticulum (ER) stress proteins, oxidative stress and heat shock proteins, and nuclear proteins involved in signaling transduction. ROLE OF TARGET IDENTIFICATION AND TARGET VALIDATION IN DRUG DISCOVERY Presented by:- Pallavi Duggal M.Pharmacy (2nd Semester) Department of Pharmacology ISF … Microarrays for gene expression have made a profound impact in the pharmaceutical and biomedical worlds. Identifying the drug target The majority of available drugs have protein molecules as their targets. 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